Author: Site Editor Publish Time: 08-07-2026 Origin: Site
In recent years, polynucleotide (PN) injection therapy has developed rapidly in the field of regenerative aesthetics, providing an effective approach for skin repair and tissue regeneration. However, it is important to recognize that although PN injection is a non-surgical or minimally invasive procedure, proper knowledge of injection techniques and treatment protocols is essential for achieving consistent clinical outcomes.
To avoid potential mistakes, having a thorough understanding of how to inject polynucleotides is essential and something every practitioner must master. After reading this guide, you will gain a clearer understanding of the PN injection process.
Polynucleotides (PN) are purified DNA fragments, typically extracted from salmon sources, used in regenerative aesthetic treatments. After injection, PN supports fibroblast activity, promotes the production of collagen, elastin, and extracellular matrix components, and helps create a regenerative environment for gradual skin improvement.
Results develop gradually, with initial improvements usually appearing within 2–4 weeks and more noticeable changes around 3 months after treatment.
The following parameters are provided as general reference examples based on PNPURE™ protocols. Actual injection depth, technique, and dosage should be adjusted by qualified practitioners according to patient anatomy and clinical assessment.
Before starting PN injection therapy, practitioners should conduct a consultation, assess and document the patient’s skin condition, and obtain informed consent.
Typical preparation includes:
Marking treatment areas such as the tear trough, cheeks, or jawline.
Thoroughly cleansing and disinfecting the treatment area.
Applying topical anesthesia when required. Most patients tolerate PN injections well after using topical numbing cream, usually for at least 30 minutes before treatment.
PN injections should only be performed by qualified medical professionals.
Different PN products may vary in concentration, viscosity, and molecular characteristics. The following approach is based on PNPURE™ protocols.
Facial rejuvenation — PNPURE™ NeoSkin
Injection plane: superficial to mid dermis
Injection depth: approximately 0.8–1.0 mm, depending on skin thickness
Needle: 30G
Injection amount: approximately 0.02–0.03 mL per injection point
Technique: multiple small injections to ensure even distribution
Facial hydration — PNPURE™ NeoBalance
The injection protocol is similar to PNPURE™ NeoSkin, with the difference that a 30–32G needle can be selected based on treatment needs and the patient’s skin condition.
Periorbital rejuvenation — PNPURE™ NeoEye
Injection plane: superficial to mid dermis
Injection depth: approximately 0.5–1.0 mm, depending on skin thickness
Needle: 32G
Injection amount: approximately 0.02–0.03 mL per injection point, with a total volume of 0.3–0.5 mL per eye per treatment session
Technique: due to thinner skin and complex anatomy around the eyes, practitioners should use a shallow and controlled injection approach.
PN works through gradual biological stimulation, so a single session may not provide the full regenerative effect.
A common clinical approach includes:
Initial course: 2–3 sessions with approximately 3–4 week intervals.
Maintenance: additional treatments every 4–6 months depending on patient condition and clinical assessment.
Treatment schedules should be adjusted according to skin quality, treatment goals, and practitioner evaluation.
After PN injection therapy, results develop gradually as skin regeneration continues.
During the first 0–72 hours, patients may experience mild redness, swelling, tightness, or small needle-point bruises in the treated area. These reactions are generally expected after injection.
Over the following 2–4 weeks, fibroblast activity increases and collagen-related remodeling processes begin. Patients may notice improvements in fine lines, skin texture, and skin elasticity.
Around 3 months after treatment, the overall improvement becomes more noticeable. Practitioners may use clinical assessment and comparison photos to evaluate treatment progress.
Time After Treatment | Care Recommendations |
Within 24 hours | Avoid water contact, makeup, and sunscreen on injection points. If cold compresses are needed, apply them with sterile gauze as a barrier and avoid direct pressure. |
24–48 hours after treatment | Gentle cleansing with water is acceptable. Avoid rubbing the treated area, sauna, steam treatments, high-temperature yoga, and excessive sun exposure. |
Within 1 week | Avoid irritating skincare products, including retinol, high-concentration acids, and pure vitamin C. Daily physical sun protection is recommended. |
When to contact a practitioner | Seek medical advice if increasing pain, progressive redness, warmth, severe swelling, or unusual skin color changes occur in the treated area. |
The clinical value of PN has been increasingly recognized, but its benefits ultimately depend on standardized treatment practices.
The above covers the key aspects of PN injection therapy. If you encounter specific clinical situations or would like to learn more about PNPURE™ application protocols, please feel free to contact us for further discussion.
