Author: Site Editor Publish Time: 06-02-2026 Origin: Site
Botulax is a highly regarded Korean botulinum toxin brand, known for its effectiveness in both cosmetic and medical applications. One of the critical aspects of using Botulax is understanding its dilution process. Botulax dilution and reconstitution require accurate calculation of the diluent volume and the resulting concentration. This page provides a practical Botulax dilution chart for 100U, 200U, and 300U presentations, including saline volume and resulting concentration references.
The chart is intended as a professional reference and should be used together with the current product labeling, manufacturer instructions, local regulations, and the clinical protocol applicable to the specific Botulax presentation. Because botulinum toxin products are not interchangeable on a unit-for-unit basis, dilution information for another botulinum toxin should not be used to calculate Botulax doses.
Botulax is a botulinum toxin type A product used to reduce facial wrinkles and treat various medical conditions such as muscle spasms, excessive sweating, and migraines. It works by relaxing the muscles that cause wrinkles, giving the skin a smoother and more youthful appearance. Botulax is available in different unit options, including 100, 200, and 300 units, each requiring specific dilution techniques.
For more information on Botulax and its applications, or to place an order, please visit our website or contact our customer service team. We are committed to providing high-quality products and support to practitioners in the medical aesthetics industry. Buy Botulax online today to experience its benefits firsthand.
Clostridium Botulinum Toxin Type A
Human Serum Albumin
Sodium Chloride
The volume of diluent added to a botulinum toxin vial determines the concentration of the reconstituted solution. For example, the same vial strength can produce different units per 0.1 mL when different volumes of diluent are used.
For this reason, dilution should always be considered together with the vial strength, the resulting concentration, the intended injection volume, and the manufacturer's instructions. Accurate calculation and consistent documentation can help reduce preparation errors and improve reproducibility between treatment sessions.
The Botulax dilution chart is a valuable tool for practitioners, providing guidelines on how to dilute the product correctly. Here is a standard dilution chart for Botulax:
Units of Botulax | Volume of Saline (ml) | Final Concentration (U/ml) |
Botulax 100 units | 2.5 ml | 40 U/ml |
Botulax 200 units | 5.0 ml | 40 U/ml |
Botulax 300 units | 7.5 ml | 40 U/ml |
How to read the concentration:
A concentration of 40 U/mL corresponds mathematically to 4 U per 0.1 mL. The units-per-0.1-mL value changes when a different reconstitution volume is used.
This chart ensures that practitioners achieve a consistent concentration of 40 units per milliliter, which is commonly used for various treatments.
Botulax 200 unit comes in a vial containing a fine, freeze-dried powder containing 200 units of Botulinum toxin type A that needs to be diluted with 0.9% saline.
Note: The toxin powder is very fine, so it could appear as if your vial is empty. Rest assured that all of your units are accounted for.
Follow the following steps to dilute & reconstitute your toxin
1. Take the vial out of the refrigerator and visually inspect: the vial should be colorless and transparent with a small quantity of white/light yellow freeze-dried powder.
2. Using the table below, draw Saline (0.9% Sodium Chloride) into a syringe and slowly add it to the Botulax 200U vial.
3. Gently and slowly swirl the vial to mix, do not shake excessively.
4. The reconstituted product should be kept in the refrigerator (2-8℃ / 35.6~46.4℉) and used within 24 hours.
BOTULAX® 100U | BOTULAX® 200U | BOTULAX® 300U | |||
Diluent added(0.9% sodium chloride injection) | Resulting dose(U/0.1ml) | Diluent added(0.9% sodium chloride injection) | Resulting dose(U/0.1ml) | Diluent added(0.9% sodium chloride injection) | Resulting dose(U/0.1ml) |
1.0mL | 10.0U | 1.0mL | 20.0U | 1.0mL | 30U |
2.0mL | 5.0U | 2.0mL | 10.0U | 2.0mL | 15U |
4.0mL | 2.5U | 4.0mL | 5.0U | 4.0mL | 7.5U |
8.0mL | 1.25U | 8.0mL | 2.5U | 8.0mL | 5U |

• Accuracy: Always use precise measurements when diluting Botulax to ensure the correct concentration.
• Sterility: Maintain a sterile environment to prevent contamination, which can compromise the product's safety and effectiveness.
• Patient Assessment: Tailor the dilution and dosage based on the patient's needs and the treatment area. Different areas may require different concentrations.
• Training: Ensure that all practitioners are adequately trained in the dilution and administration of Botulax to provide the best outcomes for patients.
• Document the preparation: Record the vial strength, reconstitution volume, preparation time, and relevant product information according to clinic protocol.
• Follow current product information: Storage conditions, in-use time, and handling requirements should always be verified against the current manufacturer instructions and applicable local requirements.
Understanding and correctly applying Botulax dilution is essential for achieving optimal results in both cosmetic and medical treatments. By following the Botulax dilution chart and adhering to best practices, practitioners can ensure the safety and satisfaction of their patients. Whether you are addressing facial wrinkles or treating medical conditions, proper dilution is the key to unlocking the full potential of Botulax.
A common dilution method is to add 7.5 mL of preservative-free 0.9% saline to a 300U vial, producing a concentration of 40 U/mL. However, practitioners may adjust the dilution based on treatment goals and injection techniques.
Many practitioners add 5 mL of saline to a 200U vial to achieve a concentration of 40 U/mL. Alternative dilution ratios can also be used depending on the desired dose per injection point.
Reconstituted Botulax should generally be stored at 2–8°C and used according to the manufacturer's recommendations. Many clinics prefer to use the product within 24 hours after reconstitution.
Most practitioners use preservative-free 0.9% sodium chloride for reconstitution. Always follow local regulations and manufacturer guidance when selecting a diluent.
No. Botulinum toxin potency units are product-specific and should not be treated as directly interchangeable between different botulinum toxin preparations. Product-specific labeling and professional guidance should be used when determining dilution and dosing.
This reference is intended for trained healthcare professionals and should be used together with the current Botulax product information supplied for the applicable market.
Reconstitution and storage information should be verified against the latest manufacturer documentation before clinical use. Because botulinum toxin potency units are product-specific, dilution or dosing information from other botulinum toxin products should not be directly substituted for Botulax information.
Last reviewed: September 2026
